Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A4 (S100A4) Standard Curve.)
Mouse Protein S100-A4 (S100A4) ELISA Kit | S100A4 elisa kit
Mouse Protein S100-A4 (S100A4) ELISA Kit
Gene Names
S100a4; 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
Reactivity
Mouse
Synonyms
Protein S100-A4 (S100A4); Mouse Protein S100-A4 (S100A4) ELISA Kit; S100A4; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; OTTHUMP00000015469; OTTHUMP00000032895; S100 calcium binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog); S100 calcium-bind; S100A4 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein S100-A4 (S100A4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S100A4. No significant cross-reactivity or interference between Mouse S100A4 and analogues was observed.
Sequence Length
101
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A4 (S100A4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A4 (S100A4) Standard Curve.)
Related Product Information for S100A4 elisa kit
Description: This Mouse Protein S100-A4 (S100A4) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A4 in samples. An antibody specific for S100A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: S100 calcium binding protein A4 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Background: S100 calcium binding protein A4 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100a4
NCBI Official Synonym Symbols
42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
NCBI Protein Information
protein S100-A4
UniProt Protein Name
Protein S100-A4
Protein Family
UniProt Gene Name
S100a4
UniProt Synonym Gene Names
Capl; Mts1
UniProt Entry Name
S10A4_MOUSE
Uniprot Description
S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Protein type: Motility/polarity/chemotaxis
Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus
Molecular Function: identical protein binding; protein binding; RAGE receptor binding; metal ion binding; calcium ion binding; actin binding; calcium-dependent protein binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade
Research Articles on S100A4
Similar Products
Product Notes
The Mouse S100A4 s100a4 (Catalog #AAA9717098) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717098 ELISA Kit recognizes Mouse S100A4. It is sometimes possible for the material contained within the vial of "Protein S100-A4 (S100A4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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