Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein dispatched homolog 2 (DISP2) Standard Curve.)
Mouse Protein Dispatched Homolog 2 (DISP2) ELISA Kit | DISP2 elisa kit
Mouse Protein Dispatched Homolog 2 (DISP2) ELISA Kit
Gene Names
Disp2; DispB; AI840597; mKIAA1742; B230210L08Rik
Reactivity
Mouse
Synonyms
Protein Dispatched Homolog 2 (DISP2); Mouse Protein Dispatched Homolog 2 (DISP2) ELISA Kit; DISP2; DISPB; DKFZp547N223; HsT16908; KIAA1742; dispatched B; DISP2 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DISP2. No significant cross-reactivity or interference between Mouse DISP2 and analogues was observed.
Sequence Length
1345
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein dispatched homolog 2 (DISP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein dispatched homolog 2 (DISP2) Standard Curve.)
Related Product Information for DISP2 elisa kit
Description: This Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit employs a two-site sandwich ELISA to quantitate DISP2 in samples. An antibody specific for DISP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DISP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DISP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched.Ma et al. (2002) isolated cDNAs encoding 2 murine homologs of dipatched, which they called Dispa and Dispb. They predicted that the dispatched proteins all have 12 transmembrane spans with cytoplasmic N and C termini.
Background: The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched.Ma et al. (2002) isolated cDNAs encoding 2 murine homologs of dipatched, which they called Dispa and Dispb. They predicted that the dispatched proteins all have 12 transmembrane spans with cytoplasmic N and C termini.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein dispatched homolog 2
NCBI Official Synonym Full Names
dispatched RND tramsporter family member 2
NCBI Official Symbol
Disp2
NCBI Official Synonym Symbols
DispB; AI840597; mKIAA1742; B230210L08Rik
NCBI Protein Information
protein dispatched homolog 2
UniProt Protein Name
Protein dispatched homolog 2
Protein Family
UniProt Gene Name
Disp2
UniProt Synonym Gene Names
Kiaa1742
UniProt Entry Name
DISP2_MOUSE
NCBI Description
The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. [provided by RefSeq, Sep 2015]
Research Articles on DISP2
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Product Notes
The Mouse DISP2 disp2 (Catalog #AAA9717877) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717877 ELISA Kit recognizes Mouse DISP2. It is sometimes possible for the material contained within the vial of "Protein Dispatched Homolog 2 (DISP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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