Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 2 (PROKR2) Standard Curve.)
Mouse Prokineticin Receptor 2 (PROKR2) ELISA Kit | PROKR2 elisa kit
Mouse Prokineticin Receptor 2 (PROKR2) ELISA Kit
Gene Names
Prokr2; PKR2; Gpr73l1; Gpcr73l1; EG-VEGRF2; B830005M06Rik
Reactivity
Mouse
Synonyms
Prokineticin Receptor 2 (PROKR2); Mouse Prokineticin Receptor 2 (PROKR2) ELISA Kit; PROKR2; GPR73L1; GPR73b; GPRg2; KAL3; PKR2; dJ680N4.3; G protein-coupled receptor 73-like 1; PROKR2 elisa kit
Reactivity
Mouse
Specificity
Mouse Prokineticin receptor 2 (PROKR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROKR2. No significant cross-reactivity or interference between Mouse PROKR2 and analogues was observed.
Sequence Length
381
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 2 (PROKR2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 2 (PROKR2) Standard Curve.)
Related Product Information for PROKR2 elisa kit
Description: This Mouse Prokineticin receptor 2 (PROKR2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROKR2 in samples. An antibody specific for PROKR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROKR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROKR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROKR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor for prokineticins. The encoded protein is similar in sequence to GPR73, another G protein-coupled receptor for prokineticins. Lin et al. (2002) cloned human GPR73L1 (PROKR2) by PCR of a pooled testis and fetal brain cDNA library. They also cloned GPR73 (PROKR1), which shares 85% overall sequence identity with GPR73L1. The greatest divergence between the proteins is in the N terminus, and both share about 80% sequence identity with mouse Gpr73. RT-PCR detected expression of GPR73L1 in brain, testis, small intestine, ovary, thyroid, pituitary, and salivary gland.
Background: Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor for prokineticins. The encoded protein is similar in sequence to GPR73, another G protein-coupled receptor for prokineticins. Lin et al. (2002) cloned human GPR73L1 (PROKR2) by PCR of a pooled testis and fetal brain cDNA library. They also cloned GPR73 (PROKR1), which shares 85% overall sequence identity with GPR73L1. The greatest divergence between the proteins is in the N terminus, and both share about 80% sequence identity with mouse Gpr73. RT-PCR detected expression of GPR73L1 in brain, testis, small intestine, ovary, thyroid, pituitary, and salivary gland.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prokineticin receptor 2
NCBI Official Synonym Full Names
prokineticin receptor 2
NCBI Official Symbol
Prokr2
NCBI Official Synonym Symbols
PKR2; Gpr73l1; Gpcr73l1; EG-VEGRF2; B830005M06Rik
NCBI Protein Information
prokineticin receptor 2
UniProt Protein Name
Prokineticin receptor 2
Protein Family
UniProt Gene Name
Prokr2
UniProt Synonym Gene Names
Gpr73l1; Pkr2; PK-R2
UniProt Entry Name
PKR2_MOUSE
Research Articles on PROKR2
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Product Notes
The Mouse PROKR2 prokr2 (Catalog #AAA9717258) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717258 ELISA Kit recognizes Mouse PROKR2. It is sometimes possible for the material contained within the vial of "Prokineticin Receptor 2 (PROKR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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