Mouse pro-opiomelanocortin, POMC ELISA Kit | POMC elisa kit

Mouse pro-opiomelanocortin, POMC ELISA Kit

Cat. Num. AAA731846

• Gene Names: POMC; LPH; MSH; NPP; POC; ACTH; CLIP
• Reactivity: Mouse
• Synonyms: pro-opiomelanocortin; POMC; Mouse pro-opiomelanocortin; POMC ELISA Kit; POMC ELISA Kit (Mouse); POMC elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This POMC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse POMC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POMC elisa kit

Principle of the assay: POMC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-POMC antibody and an POMC-HRP conjugate. The assay sample and buffer are incubated together with POMC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POMC concentration since POMC from samples and POMC-HRP conjugate compete for the anti-POMC antibody binding site. Since the number of sites is limited, as more sites are occupied by POMC from the sample, fewer sites are left to bind POMC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POMC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for POMC elisa kit

Mouse ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 49456609
• NCBI GeneID: 5443
• UniProt Accession #: Q6FHC8; P01189; Q53T23; Q53WY7; Q5TZZ7; Q6LDP0
• Molecular Weight: 29,410 Da
• NCBI Official Full Name: POMC
• NCBI Official Synonym Full Names: proopiomelanocortin
• NCBI Official Symbol: POMC
• NCBI Official Synonym Symbols: LPH; MSH; NPP; POC; ACTH; CLIP
• NCBI Protein Information: pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; OTTHUMP00000119991; OTTHUMP00000200964; OTTHUMP00000200965; OTTHUMP00000200966; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide
• UniProt Protein Name: POMC protein
• Protein Family: Pro-opiomelanocortin
• UniProt Gene Name: POMC
• UniProt Entry Name: Q6FHC8_HUMAN

NCBI Description

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Uniprot Description

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: extracellular space; peroxisomal matrix; cytoplasm; extracellular region; peroxisome; secretory granule

Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; hormone activity; type 4 melanocortin receptor binding; receptor binding

Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; peptide hormone processing; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; glucose homeostasis; signal transduction; cellular pigmentation

Disease: Obesity; Proopiomelanocortin Deficiency