Mouse Podocin (PDCN) ELISA Kit | PDCN elisa kit

Mouse Podocin (PDCN) ELISA Kit

Cat. Num. AAA2702729

• Gene Names: NPHS2; PDCN; SRN1
• Reactivity: Mouse
• Synonyms: Podocin (PDCN); Mouse Podocin (PDCN) ELISA Kit; NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant; PDCN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Podocin (PDCN).; No significant cross-reactivity or interference between Podocin (PDCN) and analogues was observed.
• Sequence Length: 315

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Podocin (PDCN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Podocin (PDCN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDCN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocin (PDCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podocin (PDCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocin (PDCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Podocin (PDCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7363002
• NCBI GeneID: 7827
• UniProt Accession #: Q9NP85; Q8N6Q5; B1AM32; B1AM33
• Molecular Weight: 34,421 Da
• NCBI Official Full Name: podocin
• NCBI Official Synonym Full Names: NPHS2, podocin
• NCBI Official Symbol: NPHS2
• NCBI Official Synonym Symbols: PDCN; SRN1
• NCBI Protein Information: podocin
• UniProt Protein Name: Podocin
• UniProt Gene Name: NPHS2

NCBI Description

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.