Typical Testing Data/Standard Curve (for reference only)
Mouse PDZK1-interacting protein 1 (PDZK1IP1) ELISA Kit | PDZK1IP1 elisa kit
Mouse PDZK1-interacting protein 1 (PDZK1IP1) ELISA Kit
Gene Names
PDZK1IP1; DD96; SPAP; MAP17
Reactivity
Mouse
Synonyms
PDZK1-interacting protein 1 (PDZK1IP1); Mouse PDZK1-interacting protein 1 (PDZK1IP1) ELISA Kit; PDZK1IP1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of PDZK1IP1. No significant cross-reactivity or interference between PDZK1IP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDZK1IP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDZK1IP1 elisa kit
Intended Uses: This PDZK1IP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PDZK1IP1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PDZK1IP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PDZK1IP1 antibody and an PDZK1IP1-HRP conjugate. The assay sample and buffer are incubated together with PDZK1IP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDZK1IP1 concentration since PDZK1IP1 from samples and PDZK1IP1-HRP conjugate compete for the anti-PDZK1IP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDZK1IP1 from the sample, fewer sites are left to bind PDZK1IP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDZK1IP1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PDZK1IP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PDZK1IP1 antibody and an PDZK1IP1-HRP conjugate. The assay sample and buffer are incubated together with PDZK1IP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDZK1IP1 concentration since PDZK1IP1 from samples and PDZK1IP1-HRP conjugate compete for the anti-PDZK1IP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDZK1IP1 from the sample, fewer sites are left to bind PDZK1IP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDZK1IP1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PDZK1IP1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,227 Da
NCBI Official Full Name
PDZK1-interacting protein 1
NCBI Official Synonym Full Names
PDZK1 interacting protein 1
NCBI Official Symbol
PDZK1IP1
NCBI Official Synonym Symbols
DD96; SPAP; MAP17
NCBI Protein Information
PDZK1-interacting protein 1; membrane-associated protein 17; 17 kDa membrane-associated protein; epithelial protein up-regulated in carcinoma, membrane associated protein 17
UniProt Protein Name
PDZK1-interacting protein 1
Protein Family
UniProt Gene Name
PDZK1IP1
UniProt Synonym Gene Names
MAP17
UniProt Entry Name
PDZ1I_HUMAN
Uniprot Description
PDZK1IP1: May play an important role in tumor biology.
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p33
Cellular Component: integral to membrane
Research Articles on PDZK1IP1
Similar Products
Product Notes
The Mouse PDZK1IP1 pdzk1ip1 (Catalog #AAA7204119) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7204119 ELISA Kit recognizes Mouse PDZK1IP1. It is sometimes possible for the material contained within the vial of "PDZK1-interacting protein 1 (PDZK1IP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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