Mouse Pdyn (Proenkephalin-B) ELISA Kit | Pdyn elisa kit

Mouse Pdyn (Proenkephalin-B) ELISA Kit

Cat. Num. AAA7606564

• Gene Names: PDYN; ADCA; PENKB; SCA23
• Reactivity: Mouse
• Synonyms: Pdyn (Proenkephalin-B); Mouse Pdyn (Proenkephalin-B) ELISA Kit; Pdyn elisa kit

• Reactivity: Mouse
• Sequence Length: 254

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pdyn elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Pdyn antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Pdyn antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pdyn amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Pdyn can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 300116232
• NCBI GeneID: 5173
• NCBI Accession #: NP_001177828.1
• NCBI GenBank Nucleotide #: NM_001190899.2
• UniProt Accession #: P01213; A8K0Q3
• Molecular Weight: 28,385 Da
• NCBI Official Full Name: proenkephalin-B preproprotein
• NCBI Official Synonym Full Names: prodynorphin
• NCBI Official Symbol: PDYN
• NCBI Official Synonym Symbols: ADCA; PENKB; SCA23
• NCBI Protein Information: proenkephalin-B
• UniProt Protein Name: Proenkephalin-B
• Protein Family: Proenkephalin
• UniProt Gene Name: PDYN
• UniProt Synonym Gene Names: Big Dyn; Dyn-A17; Dynorphin A; Dyn-B

NCBI Description

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

PDYN: Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cell soma; dendrite; extracellular region; nerve terminal; plasma membrane

Molecular Function: neuropeptide hormone activity; opioid receptor binding

Biological Process: neuropeptide signaling pathway; sensory perception; synaptic transmission

Disease: Spinocerebellar Ataxia 23