Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Parathymosin (PTMS) Standard Curve.)
Mouse Parathymosin (PTMS) ELISA Kit | PTMS elisa kit
Mouse Parathymosin (PTMS) ELISA Kit
Gene Names
Ptms; 2610009E16Rik
Reactivity
Mouse
Synonyms
Parathymosin (PTMS); Mouse Parathymosin (PTMS) ELISA Kit; PTMS; ParaT; PTMS elisa kit
Reactivity
Mouse
Specificity
Mouse Parathymosin (PTMS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTMS. No significant cross-reactivity or interference between Mouse PTMS and analogues was observed.
Sequence Length
101
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Parathymosin (PTMS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Parathymosin (PTMS) Standard Curve.)
Related Product Information for PTMS elisa kit
Description: This Mouse Parathymosin (PTMS) ELISA Kit employs a two-site sandwich ELISA to quantitate PTMS in samples. An antibody specific for PTMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Parathymosin is a polypeptide similar in size and amino acid composition to prothymosin-alpha. It has a high content of dicarboxylic amino acids and a complete absence of aromatic and sulfur-containing amino acids. It has 101 amino acid residues as compared to 111 for prothymosin. Clinton et al. (1989) reported the isolation of a cDNA clone for human kidney parathymosin containing the complete coding region and extending into the 5-prime and 3-prime flanking sequences. The open reading frame contains 306 nucleotides, including the codon for the initiator methionine. Analysis of the 5-prime flanking sequence excluded the presence of a hydrophobic signal peptide in the translated sequence. This permitted the conclusion that parathymosin, like prothymosin-alpha, is synthesized without formation of a larger precursor polypeptide.
Background: Parathymosin is a polypeptide similar in size and amino acid composition to prothymosin-alpha. It has a high content of dicarboxylic amino acids and a complete absence of aromatic and sulfur-containing amino acids. It has 101 amino acid residues as compared to 111 for prothymosin. Clinton et al. (1989) reported the isolation of a cDNA clone for human kidney parathymosin containing the complete coding region and extending into the 5-prime and 3-prime flanking sequences. The open reading frame contains 306 nucleotides, including the codon for the initiator methionine. Analysis of the 5-prime flanking sequence excluded the presence of a hydrophobic signal peptide in the translated sequence. This permitted the conclusion that parathymosin, like prothymosin-alpha, is synthesized without formation of a larger precursor polypeptide.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
parathymosin
NCBI Official Synonym Full Names
parathymosin
NCBI Official Symbol
Ptms
NCBI Official Synonym Symbols
2610009E16Rik
NCBI Protein Information
parathymosin
UniProt Protein Name
Parathymosin
Protein Family
UniProt Gene Name
Ptms
Uniprot Description
Parathymosin may mediate immune function by blocking the effect of prothymosin alpha which confers resistance to certain opportunistic infections.
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Product Notes
The Mouse PTMS ptms (Catalog #AAA9717227) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717227 ELISA Kit recognizes Mouse PTMS. It is sometimes possible for the material contained within the vial of "Parathymosin (PTMS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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