Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pancreatic prohormone (PPY) Standard Curve.)
Mouse Pancreatic Prohormone (PPY) ELISA Kit | PPY elisa kit
Mouse Pancreatic Prohormone (PPY) ELISA Kit
Gene Names
Ppy; PP; IGSPP; AV061413
Reactivity
Mouse
Synonyms
Pancreatic Prohormone (PPY); Mouse Pancreatic Prohormone (PPY) ELISA Kit; PPY; PNP; pancreatic polypeptide Y; PPY elisa kit
Reactivity
Mouse
Specificity
Mouse Pancreatic prohormone (PPY) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPY. No significant cross-reactivity or interference between Mouse PPY and analogues was observed.
Sequence Length
100
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pancreatic prohormone (PPY) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pancreatic prohormone (PPY) Standard Curve.)
Related Product Information for PPY elisa kit
Description: This Mouse Pancreatic prohormone (PPY) ELISA Kit employs a two-site sandwich ELISA to quantitate PPY in samples. An antibody specific for PPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pancreatic polypeptide (PP) is a 36-amino-acid secretory peptide that is predominantly produced by the pancreas. The exact physiologic role of PP in healthy individuals has not been fully defined. This peptide affects the secretion of pancreatic enzymes, water, and electrolytes. Its effect is biphasic in that PP initially enhances secretion and then inhibits secretion. PP increases gastric emptying and gut motility. It also relaxes the pyloric and ileocecocolic sphincters, the colon, and gallbladder. PP levels increase after ngestion of food and remain elevated from 4-8 hours. Prolonged fasting, diabetes, and exercise can also increase PP levels. Serum PP levels can be elevated in as many as 50% of patients with carcinoid syndrome. Increased levels can also be found in patients with duodenal ulcers and in patients with type I diabetes.
Background: Pancreatic polypeptide (PP) is a 36-amino-acid secretory peptide that is predominantly produced by the pancreas. The exact physiologic role of PP in healthy individuals has not been fully defined. This peptide affects the secretion of pancreatic enzymes, water, and electrolytes. Its effect is biphasic in that PP initially enhances secretion and then inhibits secretion. PP increases gastric emptying and gut motility. It also relaxes the pyloric and ileocecocolic sphincters, the colon, and gallbladder. PP levels increase after ngestion of food and remain elevated from 4-8 hours. Prolonged fasting, diabetes, and exercise can also increase PP levels. Serum PP levels can be elevated in as many as 50% of patients with carcinoid syndrome. Increased levels can also be found in patients with duodenal ulcers and in patients with type I diabetes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pancreatic prohormone
NCBI Official Synonym Full Names
pancreatic polypeptide
NCBI Official Symbol
Ppy
NCBI Official Synonym Symbols
PP; IGSPP; AV061413
NCBI Protein Information
pancreatic prohormone
UniProt Protein Name
Pancreatic prohormone
Protein Family
UniProt Gene Name
Ppy
UniProt Synonym Gene Names
PP; PH
Uniprot Description
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
Research Articles on PPY
Similar Products
Product Notes
The Mouse PPY ppy (Catalog #AAA9718045) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718045 ELISA Kit recognizes Mouse PPY. It is sometimes possible for the material contained within the vial of "Pancreatic Prohormone (PPY), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
