Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neurocalcin-delta (NCALD) Standard Curve.)
Mouse Neurocalcin-delta (NCALD) ELISA Kit | NCALD elisa kit
Mouse Neurocalcin-delta (NCALD) ELISA Kit
Gene Names
Ncald; AI848120; D15Ertd412e; D030020D09Rik
Reactivity
Mouse
Synonyms
Neurocalcin-delta (NCALD); Mouse Neurocalcin-delta (NCALD) ELISA Kit; MGC33870; MGC74858; NCALD elisa kit
Reactivity
Mouse
Specificity
Mouse Neurocalcin-delta (NCALD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCALD. No significant cross-reactivity or interference between Mouse NCALD and analogues was observed.
Sequence Length
193
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neurocalcin-delta (NCALD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neurocalcin-delta (NCALD) Standard Curve.)
Related Product Information for NCALD elisa kit
Description: This Mouse Neurocalcin-delta (NCALD) ELISA Kit employs a two-site sandwich ELISA to quantitate NCALD in samples. An antibody specific for NCALD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCALD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCALD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCALD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ncald encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The neurocalcin delta contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
Background: Ncald encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The neurocalcin delta contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neurocalcin-delta
NCBI Official Synonym Full Names
neurocalcin delta
NCBI Official Symbol
Ncald
NCBI Official Synonym Symbols
AI848120; D15Ertd412e; D030020D09Rik
NCBI Protein Information
neurocalcin-delta
UniProt Protein Name
Neurocalcin-delta
Protein Family
UniProt Gene Name
Ncald
UniProt Synonym Gene Names
D15Ertd412e
UniProt Entry Name
NCALD_MOUSE
Research Articles on NCALD
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Product Notes
The Mouse NCALD ncald (Catalog #AAA9717418) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717418 ELISA Kit recognizes Mouse NCALD. It is sometimes possible for the material contained within the vial of "Neurocalcin-delta (NCALD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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