Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) Standard Curve.)
Mouse N-Acetylglucosamine-1-Phosphodiester alpha-N-Acetylglucosaminidase (NAGPA) ELISA Kit | NAGPA elisa kit
Mouse N-Acetylglucosamine-1-Phosphodiester alpha-N-Acetylglucosaminidase (NAGPA) ELISA Kit
Gene Names
Nagpa; UCE; AI596180
Reactivity
Mouse
Synonyms
N-Acetylglucosamine-1-Phosphodiester alpha-N-Acetylglucosaminidase (NAGPA); Mouse N-Acetylglucosamine-1-Phosphodiester alpha-N-Acetylglucosaminidase (NAGPA) ELISA Kit; NAGPA; APAA; UCE; alpha-N-acetylglucosaminyl phosphodiesterase; lysosomal alpha-N-acetylglucosaminidase; mannose 6-phosphate-uncovering enzyme; phosphodiester alpha-GlcNAcase; NAGPA elisa kit
Reactivity
Mouse
Specificity
Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NAGPA. No significant cross-reactivity or interference between Mouse NAGPA and analogues was observed.
Sequence Length
517
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) Standard Curve.)
Related Product Information for NAGPA elisa kit
Description: This Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) ELISA Kit employs a two-site sandwich ELISA to quantitate NAGPA in samples. An antibody specific for NAGPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAGPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAGPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAGPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. Nagpa encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients.
Background: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. Nagpa encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
NCBI Official Synonym Full Names
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
NCBI Official Symbol
Nagpa
NCBI Official Synonym Symbols
UCE; AI596180
NCBI Protein Information
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
UniProt Protein Name
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
UniProt Gene Name
Nagpa
UniProt Entry Name
NAGPA_MOUSE
Research Articles on NAGPA
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Product Notes
The Mouse NAGPA nagpa (Catalog #AAA9717443) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717443 ELISA Kit recognizes Mouse NAGPA. It is sometimes possible for the material contained within the vial of "N-Acetylglucosamine-1-Phosphodiester alpha-N-Acetylglucosaminidase (NAGPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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