Mouse Myosin Binding Protein C, Slow Type (MYBPC1) ELISA Kit | MYBPC1 elisa kit

Mouse Myosin Binding Protein C, Slow Type (MYBPC1) ELISA Kit

Cat. Num. AAA2020793

• Gene Names: MYBPC1; LCCS4; MYBPCC; MYBPCS
• Reactivity: Mouse
• Synonyms: Myosin Binding Protein C; Slow Type (MYBPC1); Mouse Myosin Binding Protein C; Slow Type (MYBPC1) ELISA Kit; MYBPCC; MYBPCS; C-protein; skeletal muscle slow isoform; MYBPC1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Binding Protein C, Slow Type (MYBPC1).; No significant cross-reactivity or interference between Myosin Binding Protein C, Slow Type (MYBPC1) and analogues was observed.
• Sequence Length: 1123

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYBPC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Binding Protein C, Slow Type (MYBPC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Binding Protein C, Slow Type (MYBPC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Binding Protein C, Slow Type (MYBPC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Binding Protein C, Slow Type (MYBPC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MYBPC1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 62203084
• NCBI GeneID: 4604
• UniProt Accession #: Q00872; Q15497; Q17RR7; Q569K7; B4DKR5; B7Z8G8; B7ZL02; B7ZL09; B7ZL10; E7ESM5; E7EWS6; G3XAE8
• Molecular Weight: 129,074 Da
• NCBI Official Full Name: Myosin binding protein C, slow type
• NCBI Official Synonym Full Names: myosin binding protein C, slow type
• NCBI Official Symbol: MYBPC1
• NCBI Official Synonym Symbols: LCCS4; MYBPCC; MYBPCS
• NCBI Protein Information: myosin-binding protein C, slow-type
• UniProt Protein Name: Myosin-binding protein C, slow-type
• Protein Family: Myosin-binding protein
• UniProt Gene Name: MYBPC1
• UniProt Synonym Gene Names: MYBPCS; Slow MyBP-C
• UniProt Entry Name: MYPC1_HUMAN

NCBI Description

This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

MYBPC1: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC1 are the cause of arthrogryposis, distal, type 1B (DA1B). A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Defects in MYBPC1 may be a cause of autosomal recessive lethal congenital contractural syndrome (LCCS), a severe, neonatally lethal form of arthrogryposis. Belongs to the immunoglobulin superfamily. MyBP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Myosin-binding; Actin-binding

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: myofibril; cytosol

Molecular Function: protein binding; structural constituent of muscle; titin binding; actin binding

Biological Process: cell adhesion; muscle filament sliding

Disease: Lethal Congenital Contracture Syndrome 4; Arthrogryposis, Distal, Type 1b