Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) ELISA Kit | MPIF2 elisa kit

Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) ELISA Kit

Cat. Num. AAA2702298

• Gene Names: CCL24; Ckb-6; MPIF2; MPIF-2; SCYA24
• Reactivity: Mouse
• Synonyms: Myeloid Progenitor Inhibitory Factor 2 (MPIF2); Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) ELISA Kit; CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24; MPIF2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2).; No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.
• Sequence Length: 119

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MPIF2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Progenitor Inhibitory Factor 2 (MPIF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Progenitor Inhibitory Factor 2 (MPIF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1916252
• NCBI GeneID: 6369
• UniProt Accession #: O00175; B2R5K2
• Molecular Weight: 13,134 Da
• NCBI Official Full Name: MPIF-2
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 24
• NCBI Official Symbol: CCL24
• NCBI Official Synonym Symbols: Ckb-6; MPIF2; MPIF-2; SCYA24
• NCBI Protein Information: C-C motif chemokine 24
• UniProt Protein Name: C-C motif chemokine 24
• UniProt Gene Name: CCL24
• UniProt Synonym Gene Names: MPIF2; SCYA24; MPIF-2

NCBI Description

This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. [provided by RefSeq, Jul 2008]

Uniprot Description

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.