Mouse Mucin-7 (MUC7) ELISA Kit | MUC7 elisa kit

Mouse Mucin-7 (MUC7) ELISA Kit

Cat. Num. AAA9718175

• Gene Names: MUC7; MG2
• Reactivity: Mouse
• Synonyms: Mucin-7 (MUC7); Mouse Mucin-7 (MUC7) ELISA Kit; MUC7 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Mucin-7 (MUC7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MUC7. No significant cross-reactivity or interference between Mouse MUC7 and analogues was observed.
• Sequence Length: 377

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Mucin-7 (MUC7) Standard Curve.)

Related Product Information for MUC7 elisa kit

Description: This Mouse Mucin-7 (MUC7) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC7 in samples. An antibody specific for MUC7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The MUC7 gene encodes a small salivary mucin, which is thought to function in a protective capacity by promoting the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing.Bobek et al. (1996) found that the MUC7 gene spans approximately 10 kb and comprises 3 exons and 2 introns. Intron 1 is approximately 1.7 kb long and is located in the 5-prime untranslated region of the MUC7 cDNA.Intron 2 spans approximately 6 kb and is located close to the boundary of the putative liter peptide and secreted protein. The entire region encoding the secreted peptide is located on exon 3, spanning approximately 2.2 kb.Bobek et al. (1993) described the isolation and characterization of overlapping cDNA clones that code for the low molecular weight glycoprotein core.

NCBI and Uniprot Product Information

• NCBI GI #: 222418645
• NCBI GeneID: 4589
• NCBI GenBank Nucleotide #: NP_689504.2
• UniProt Accession #: Q8TAX7
• NCBI Official Full Name: mucin-7
• NCBI Official Synonym Full Names: mucin 7, secreted
• NCBI Official Symbol: MUC7
• NCBI Official Synonym Symbols: MG2
• NCBI Protein Information: mucin-7
• Protein Family: Mucin

NCBI Description

This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Oct 2014]