Mouse Metabotropic glutamate receptor 2 (GRM2) ELISA Kit | GRM2 elisa kit

Mouse Metabotropic glutamate receptor 2 (GRM2) ELISA Kit

Cat. Num. AAA7223774

• Gene Names: GRM2; GLUR2; mGlu2; GPRC1B; MGLUR2
• Reactivity: Mouse
• Synonyms: Metabotropic glutamate receptor 2 (GRM2); Mouse Metabotropic glutamate receptor 2 (GRM2) ELISA Kit; GRM2 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRM2 elisa kit

Intended Uses: This GRM2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GRM2.

Principle of the Assay: GRM2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-GRM2 antibody and an GRM2-HRP conjugate. The assay sample and buffer are incubated together with GRM2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRM2 concentration since GRM2 from samples and GRM2-HRP conjugate compete for the anti-GRM2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRM2 from the sample, fewer sites are left to bind GRM2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRM2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GRM2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 66529100
• NCBI GeneID: 2912
• NCBI Accession #: NP_000830.2
• NCBI GenBank Nucleotide #: NM_000839.3
• Molecular Weight: 95,568 Da
• NCBI Official Full Name: metabotropic glutamate receptor 2 isoform a
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 2
• NCBI Official Symbol: GRM2
• NCBI Official Synonym Symbols: GLUR2; mGlu2; GPRC1B; MGLUR2
• NCBI Protein Information: metabotropic glutamate receptor 2; glutamate receptor homolog; glutamate metabotropic receptor 2
• UniProt Protein Name: Metabotropic glutamate receptor 2
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM2
• UniProt Synonym Gene Names: GPRC1B; MGLUR2; mGluR2
• UniProt Entry Name: GRM2_HUMAN

NCBI Description

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

mGluR2: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization. Belongs to the G-protein coupled receptor 3 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 3

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: presynaptic membrane; integral to plasma membrane; axon; dendrite; plasma membrane; intracellular; cell junction

Molecular Function: G-protein coupled receptor activity; group II metabotropic glutamate receptor activity; calcium channel regulator activity; glutamate receptor activity

Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; glutamate secretion; negative regulation of adenylate cyclase activity; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway