Mouse M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit | M-CSF elisa kit

Mouse M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit

Cat. Num. AAA7606659

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Reactivity: Mouse
• Synonyms: M-CSF (Macrophage Colony-Stimulating Factor); Mouse M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit; M-CSF; MCSF; CSF1; CSF-1; M-CSF elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection ofMcpt8. No significant cross-reactivity or interference betweenMcpt8 and analogues was observed.
• Sequence Length: 552

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.12-200mIU/ml
• Sensitivity: <1.875mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelMcpt8 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelMcpt8 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for M-CSF elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mcpt8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mcpt8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mcpt8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMcpt8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1345843
• NCBI GeneID: 12977
• UniProt Accession #: P07141; Q3U395; Q8R3C8
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: Macrophage colony-stimulating factor 1
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3 F2.3|3 46.83 cM

Cellular Component: extracellular space; perinuclear region of cytoplasm; plasma membrane

Molecular Function: cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding; protein binding; protein homodimerization activity

Biological Process: cell proliferation; homeostasis of number of cells within a tissue; macrophage differentiation; odontogenesis; ossification; osteoclast differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of mononuclear cell proliferation; positive regulation of multicellular organism growth; positive regulation of odontogenesis of dentine-containing teeth; positive regulation of osteoclast differentiation; positive regulation of protein kinase activity; positive regulation of Ras protein signal transduction; regulation of ossification; reproductive developmental process; transmembrane receptor protein tyrosine kinase signaling pathway