Mouse Lysophosphatidic Acid Receptor 3 (LPAR3) ELISA Kit | LPAR3 elisa kit

Mouse Lysophosphatidic Acid Receptor 3 (LPAR3) ELISA Kit

Cat. Num. AAA2705838

• Gene Names: LPAR3; EDG7; GPCR; LPA3; Edg-7; LP-A3; HOFNH30; RP4-678I3
• Reactivity: Mouse
• Synonyms: Lysophosphatidic Acid Receptor 3 (LPAR3); Mouse Lysophosphatidic Acid Receptor 3 (LPAR3) ELISA Kit; GPCR; EDG7; HOFNH30; LP-A3; LPA3; LPAR3; Endothelial Differentiation; Lysophosphatidic Acid G-Protein-Coupled Receptor 7; Lysophosphatidic acid receptor Edg-7; LPAR3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidic Acid Receptor 3 (LPAR3).; No significant cross-reactivity or interference between Lysophosphatidic Acid Receptor 3 (LPAR3) and analogues was observed.
• Sequence Length: 353

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidic Acid Receptor 3 (LPAR3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidic Acid Receptor 3 (LPAR3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPAR3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidic Acid Receptor 3 (LPAR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidic Acid Receptor 3 (LPAR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidic Acid Receptor 3 (LPAR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysophosphatidic Acid Receptor 3 (LPAR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6912348
• NCBI GeneID: 23566
• NCBI Accession #: NP_036284.1
• NCBI GenBank Nucleotide #: NM_012152.2
• UniProt Accession #: Q9UBY5; A0AVA3
• Molecular Weight: 40,128 Da
• NCBI Official Full Name: lysophosphatidic acid receptor 3
• NCBI Official Synonym Full Names: lysophosphatidic acid receptor 3
• NCBI Official Symbol: LPAR3
• NCBI Official Synonym Symbols: EDG7; GPCR; LPA3; Edg-7; LP-A3; HOFNH30; RP4-678I3
• NCBI Protein Information: lysophosphatidic acid receptor 3
• UniProt Protein Name: Lysophosphatidic acid receptor 3
• Protein Family: Lysophosphatidic acid receptor
• UniProt Gene Name: LPAR3
• UniProt Synonym Gene Names: EDG7; LPA3; LPA receptor 3; LPA-3

NCBI Description

This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. May play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins.