Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lymphocyte antigen 75 (LY75) Standard Curve.)
Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit | LY75 elisa kit
Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit
Gene Names
Ly75; CD205; DEC205; DEC-205
Reactivity
Mouse
Synonyms
Lymphocyte Antigen 75 (LY75); Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit; LY75; CD205; CLEC13B; DEC-205; GP200-MR6; LY75 elisa kit
Reactivity
Mouse
Specificity
Mouse Lymphocyte antigen 75 (LY75) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LY75. No significant cross-reactivity or interference between Mouse LY75 and analogues was observed.
Sequence Length
1723
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lymphocyte antigen 75 (LY75) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lymphocyte antigen 75 (LY75) Standard Curve.)
Related Product Information for LY75 elisa kit
Description: This Mouse Lymphocyte antigen 75 (LY75) ELISA Kit employs a two-site sandwich ELISA to quantitate LY75 in samples. An antibody specific for LY75 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LY75 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY75 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY75 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The LY75 protein has 77% overall amino acid identity with mouse Dec205. LY75 is a member of the MMR family of multidomain molecules. It contains a signal peptide, a cysteine-rich domain, a fibronectin type II domain, 10 carbohydrate recognition-like domains, a transmembrane domain, and a cytoplasmic tail. Northern blot analysis of hematopoietic cell lines revealed the presence of a predominant 7.8-kb and a secondary 9.5-kb transcript in myeloid, B-cell, and Hodgkin cell lines but not in the Jurkat T-cell line. In peripheral blood, immature DC contained barely detectable transcripts, which increased 50-fold upon differentiation and activation.LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.
Background: The LY75 protein has 77% overall amino acid identity with mouse Dec205. LY75 is a member of the MMR family of multidomain molecules. It contains a signal peptide, a cysteine-rich domain, a fibronectin type II domain, 10 carbohydrate recognition-like domains, a transmembrane domain, and a cytoplasmic tail. Northern blot analysis of hematopoietic cell lines revealed the presence of a predominant 7.8-kb and a secondary 9.5-kb transcript in myeloid, B-cell, and Hodgkin cell lines but not in the Jurkat T-cell line. In peripheral blood, immature DC contained barely detectable transcripts, which increased 50-fold upon differentiation and activation.LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lymphocyte antigen 75
NCBI Official Synonym Full Names
lymphocyte antigen 75
NCBI Official Symbol
Ly75
NCBI Official Synonym Symbols
CD205; DEC205; DEC-205
NCBI Protein Information
lymphocyte antigen 75
UniProt Protein Name
Lymphocyte antigen 75
Protein Family
UniProt Gene Name
Ly75
UniProt Synonym Gene Names
Cd205; Ly-75
UniProt Entry Name
LY75_MOUSE
Uniprot Description
LY75: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment. Causes reduced proliferation of B-lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Molecular Function: carbohydrate binding
Biological Process: endocytosis
Research Articles on LY75
Similar Products
Product Notes
The Mouse LY75 ly75 (Catalog #AAA9717756) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717756 ELISA Kit recognizes Mouse LY75. It is sometimes possible for the material contained within the vial of "Lymphocyte Antigen 75 (LY75), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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