Typical Testing Data/Standard Curve (for reference only)
Mouse Luteinizing Hormone (LH) ELISA Kit | LH elisa kit
Mouse Luteinizing Hormone (LH) ELISA Kit
Gene Names
LHB; CGB4; HH23; LSH-B; LSH-beta
Reactivity
Mouse
Synonyms
Luteinizing Hormone (LH); Mouse Luteinizing Hormone (LH) ELISA Kit; ICSH; Lutropin; Interstitial Cell Stimulating Hormone; LH elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of INHA. No significant cross-reactivity or interference between INHA and analogues was observed.
Sequence Length
5
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Competitive
Detection Range
12.35-1,000pg/mL
Sensitivity
5.11pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level INHA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level INHA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LH elisa kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of INHA in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with An antibody specific to INHA. A competitive inhibition reaction is launched between biotin labeled INHA and unlabeled INHA (Standards or samples) with the pre-coated antibody specific to INHA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of INHA in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the INHA level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with An antibody specific to INHA. A competitive inhibition reaction is launched between biotin labeled INHA and unlabeled INHA (Standards or samples) with the pre-coated antibody specific to INHA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of INHA in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the INHA level in the sample or standard.
Product Categories/Family for LH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,345 Da
NCBI Official Full Name
luteinizing hormone beta subunit (LHbeta), partial
NCBI Official Synonym Full Names
luteinizing hormone beta polypeptide
NCBI Official Symbol
LHB
NCBI Official Synonym Symbols
CGB4; HH23; LSH-B; LSH-beta
NCBI Protein Information
lutropin subunit beta
UniProt Protein Name
Lutropin subunit beta
UniProt Gene Name
LHB
UniProt Synonym Gene Names
Lutropin beta chain; LH-B; LSH-B; LSH-beta
NCBI Description
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. [provided by RefSeq, Jul 2008]
Uniprot Description
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Research Articles on LH
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Product Notes
The Mouse LH lhb (Catalog #AAA2104790) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104790 ELISA Kit recognizes Mouse LH. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the LH lhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Luteinizing Hormone (LH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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