Mouse Leukotriene C4 (LTC4) ELISA Kit | LTC4 elisa kit

Mouse Leukotriene C4 (LTC4) ELISA Kit

Cat. Num. AAA262793

• Reactivity: Mouse
• Synonyms: Leukotriene C4 (LTC4); Mouse Leukotriene C4 (LTC4) ELISA Kit; LTC4 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 150

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for LTC4 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse LTC4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 22035629
• NCBI GeneID: 4056
• NCBI Accession #: NP_665874.1
• NCBI GenBank Nucleotide #: NM_145867.1
• Molecular Weight: 16,567 Da
• NCBI Official Full Name: leukotriene C4 synthase
• NCBI Official Synonym Full Names: leukotriene C4 synthase
• NCBI Official Symbol: LTC4S
• NCBI Protein Information: leukotriene C4 synthase; LTC4 synthase
• UniProt Protein Name: Leukotriene C4 synthase
• UniProt Gene Name: LTC4S
• UniProt Synonym Gene Names: LTC4 synthase
• UniProt Entry Name: LTC4S_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]

Uniprot Description

LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: Lyase; Membrane protein, multi-pass; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; Nuclear envelope; EC 4.4.1.20; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; glutathione peroxidase activity; lipid binding

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Leukotriene C4 Synthase Deficiency