Mouse Leptin receptor, LR/Ob-R ELISA Kit | LR/Ob-R elisa kit

Mouse Leptin receptor, LR/Ob-R ELISA Kit

Cat. Num. AAA163370

• Reactivity: Mouse
• Synonyms: Leptin receptor; LR/Ob-R; Mouse Leptin receptor; LR/Ob-R ELISA Kit; LR/Ob-R elisa kit

• Reactivity: Mouse
• Sequence Length: 1165

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 30ng/ml
• Sensitivity: 0.022ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LR/Ob-R elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Androstenedione (also known as ASD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ASD antibody. ASD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ASD Antibody is added and binds to ASD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ASD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ASD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1589772
• Molecular Weight: 102,490 Da
• NCBI Official Full Name: leptin receptor
• UniProt Protein Name: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R

Uniprot Description

Receptor for hormone LEP/leptin (Probable) (PubMed:22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones () (PubMed:9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25060689, PubMed:12504075, PubMed:8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production ().