Mouse Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) ELISA Kit | LTBP1 elisa kit

Mouse Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) ELISA Kit

Cat. Num. AAA2704211

• Reactivity: Mouse
• Synonyms: Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1); Mouse Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) ELISA Kit; TGF-beta1-BP-1; Transforming growth factor beta-1-binding protein 1; LTBP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1).; No significant cross-reactivity or interference between Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) and analogues was observed.
• Sequence Length: 1,396

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTBP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 261337167
• NCBI GeneID: 4052
• NCBI Accession #: NP_000618.3
• NCBI GenBank Nucleotide #: NM_000627.3
• UniProt Accession #: Q14766; P22064; Q53SD8; Q53SF3; Q53SG1; Q59HF7; Q8TD95; A1L3V1
• Molecular Weight: 153,006 Da
• NCBI Official Full Name: latent-transforming growth factor beta-binding protein 1 isoform LTBP-1S
• NCBI Official Synonym Full Names: latent transforming growth factor beta binding protein 1
• NCBI Official Symbol: LTBP1
• NCBI Protein Information: latent-transforming growth factor beta-binding protein 1
• UniProt Protein Name: Latent-transforming growth factor beta-binding protein 1
• Protein Family: Latent-transforming growth factor beta-binding protein
• UniProt Gene Name: LTBP1
• UniProt Synonym Gene Names: LTBP-1; TGF-beta1-BP-1

NCBI Description

The protein encoded by this gene belongs to the family of latent TGF-beta binding proteins (LTBPs). The secretion and activation of TGF-betas is regulated by their association with latency-associated proteins and with latent TGF-beta binding proteins. The product of this gene targets latent complexes of transforming growth factor beta to the extracellular matrix, where the latent cytokine is subsequently activated by several different mechanisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extracellular matrix (ECM).