Mouse Laminin Alpha 1 (LAMa1) ELISA Kit | LAMa1 elisa kit

Mouse Laminin Alpha 1 (LAMa1) ELISA Kit

Cat. Num. AAA2702070

• Gene Names: LAMA1; LAMA; PTBHS; S-LAM-alpha
• Reactivity: Mouse
• Synonyms: Laminin Alpha 1 (LAMa1); Mouse Laminin Alpha 1 (LAMa1) ELISA Kit; LAM-A1; LAMA; Laminin A; S-laminin subunit alpha; LAMa1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Laminin Alpha 1 (LAMa1).; No significant cross-reactivity or interference between Laminin Alpha 1 (LAMa1) and analogues was observed.
• Sequence Length: 1

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.32ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 1 (LAMa1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 1 (LAMa1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMa1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 1 (LAMa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 1 (LAMa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 1 (LAMa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 1 (LAMa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 121945888
• NCBI GeneID: 284217
• Molecular Weight: 337,084 Da
• NCBI Official Full Name: laminin alpha 1, partial
• NCBI Official Synonym Full Names: laminin subunit alpha 1
• NCBI Official Symbol: LAMA1
• NCBI Official Synonym Symbols: LAMA; PTBHS; S-LAM-alpha
• NCBI Protein Information: laminin subunit alpha-1
• UniProt Protein Name: Laminin subunit alpha-1
• Protein Family: Laminin
• UniProt Gene Name: LAMA1
• UniProt Synonym Gene Names: LAMA; S-LAM alpha

NCBI Description

This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]

Uniprot Description

LAMA1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; laminin-1 complex; laminin-3 complex; membrane; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; protein binding; protein C-terminus binding; receptor binding

Biological Process: cell adhesion; cell surface receptor signaling pathway; extracellular matrix organization; regulation of cell adhesion; regulation of cell migration; regulation of embryonic development

Disease: Poretti-boltshauser Syndrome