Mouse Kininogen 1 (KNG1) ELISA Kit | KNG1 elisa kit

Mouse Kininogen 1 (KNG1) High Sensitive ELISA Kit

Cat. Num. AAA2708070

• Gene Names: Kng1; Kng
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Kininogen 1 (KNG1); Mouse Kininogen 1 (KNG1) High Sensitive ELISA Kit; Kininogen 1; HMWK; High Molecular Weight Kininogen; Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor; KNG1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Kininogen 1 (KNG1).; No significant cross-reactivity or interference between High Sensitive Kininogen 1 (KNG1) and analogues was observed.
• Sequence Length: 480

• Applicable Applications for KNG1 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Kininogen 1 (KNG1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Kininogen 1 (KNG1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Kininogen 1 (KNG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Kininogen 1 (KNG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Kininogen 1 (KNG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Kininogen 1 (KNG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 50082914
• NCBI GeneID: 16644
• UniProt Accession #: O08677
• NCBI Official Full Name: high molecular weight kininogen I isoform DeltaD5
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: Kng1
• NCBI Official Synonym Symbols: Kng
• NCBI Protein Information: kininogen-1
• UniProt Protein Name: Kininogen-1
• Protein Family: Kininogen
• UniProt Gene Name: Kng1
• UniProt Synonym Gene Names: Kng
• UniProt Entry Name: KNG1_MOUSE

Uniprot Description

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Cell adhesion; Secreted, signal peptide; Inhibitor; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular space; extracellular region

Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity; receptor binding

Biological Process: hemostasis; negative regulation of peptidase activity; regulation of blood vessel size; blood coagulation; inflammatory response; vasodilation