Mouse Jagged 1 Protein (JAG1) ELISA Kit | JAG1 elisa kit

Mouse Jagged 1 Protein (JAG1) ELISA Kit

Cat. Num. AAA2019463

• Gene Names: JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1
• Reactivity: Mouse
• Synonyms: Jagged 1 Protein (JAG1); Mouse Jagged 1 Protein (JAG1) ELISA Kit; CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome; JAG1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Jagged 1 Protein (JAG1).; No significant cross-reactivity or interference between Jagged 1 Protein (JAG1) and analogues was observed.
• Sequence Length: 38

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 6.0pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Jagged 1 Protein (JAG1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Jagged 1 Protein (JAG1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAG1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged 1 Protein (JAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Jagged 1 Protein (JAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jagged 1 Protein (JAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Jagged 1 Protein (JAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for JAG1 elisa kit

Signal transduction; CD & Adhesion molecule; Tumor immunity; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 215536139
• NCBI GeneID: 182
• Molecular Weight: 4,286 Da
• NCBI Official Full Name: jagged 1, partial
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: JAG1
• NCBI Official Synonym Symbols: AGS; AHD; AWS; HJ1; CD339; JAGL1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Jagged 1
• Protein Family: Protein jagged
• UniProt Gene Name: JAG1
• UniProt Entry Name: B7U6M9_HUMAN

NCBI Description

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.1-p11.23

Cellular Component: membrane; apical part of cell; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding; structural molecule activity

Biological Process: nervous system development; endothelial cell differentiation; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; myoblast differentiation; keratinocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; blood vessel remodeling; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of Notch signaling pathway

Disease: Tetralogy Of Fallot; Alagille Syndrome 1