Mouse Interleukin 34 (IL34) ELISA Kit | IL34 elisa kit
Mouse Interleukin 34 (IL34) Mini Samples ELISA Kit
Gene Names
Il34; AI593503; 2010004A03Rik
Reactivity
Mouse
Applications
ELISA
Synonyms
Interleukin 34 (IL34); Mouse Interleukin 34 (IL34) Mini Samples ELISA Kit; Interleukin 34; C16orf77; IL34 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 34 (IL34).
No significant cross-reactivity or interference between Mini Samples Interleukin 34 (IL34) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Interleukin 34 (IL34) and analogues was observed.
Sequence Length
235
Applicable Applications for IL34 elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 34 (IL34) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 34 (IL34) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 34 (IL34) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 34 (IL34). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 34 (IL34). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 34 (IL34), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 34 (IL34) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-34 isoform 1
NCBI Official Synonym Full Names
interleukin 34
NCBI Official Symbol
Il34
NCBI Official Synonym Symbols
AI593503; 2010004A03Rik
NCBI Protein Information
interleukin-34
UniProt Protein Name
Interleukin-34
Protein Family
UniProt Gene Name
Il34
UniProt Synonym Gene Names
IL-34
UniProt Entry Name
IL34_MOUSE
Uniprot Description
IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: macrophage colony stimulating factor receptor binding
Biological Process: positive regulation of cell proliferation; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of protein amino acid phosphorylation
Research Articles on IL34
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Product Notes
The Mouse IL34 il34 (Catalog #AAA2708376) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708376 ELISA Kit recognizes Mouse IL34. AAA Biotech's Interleukin 34 (IL34) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the IL34 il34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 34 (IL34), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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