Mouse Interleukin 16 (IL16) ELISA Kit | IL16 elisa kit

Mouse Interleukin 16 (IL16) ELISA Kit

Cat. Num. AAA2700986

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Reactivity: Mouse
• Synonyms: Interleukin 16 (IL16); Mouse Interleukin 16 (IL16) ELISA Kit; LCF; PrIL-16; Lymphocyte Chemoattractant Factor; Pro-Interleukin 16; IL16 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 16 (IL16).; No significant cross-reactivity or interference between Interleukin 16 (IL16) and analogues was observed.
• Sequence Length: 130

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 16 (IL16) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 16 (IL16) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL16 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 16 (IL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 16 (IL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 16 (IL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 16 (IL16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2996198
• NCBI GeneID: 3603
• Molecular Weight: 59,345 Da
• NCBI Official Full Name: interleukin-16
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16
• UniProt Protein Name: Pro-interleukin-16
• Protein Family: Pro-interleukin
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.