Mouse INO80 complex subunit B (INO80B) ELISA Kit | INO80B elisa kit

Mouse INO80 complex subunit B (INO80B) ELISA Kit

Cat. Num. AAA287586

• Gene Names: Ino80b; Papa1; Znhit4; HMG1YL4; Hmga1l4; 2510009I23Rik
• Reactivity: Mouse
• Synonyms: INO80 complex subunit B (INO80B); Mouse INO80 complex subunit B (INO80B) ELISA Kit; HMGA1L4; HMGIYL4; IES2; PAP-1BP; PAPA-1; ZNHIT4; hIes2; IES2 homolog; PAP (Pim-1 associated protein) associated protein 1; PAP-1 binding protein; high mobility group AT-hook 1-like 4; zinc finger; HIT t; INO80B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse INO80B. No significant cross-reactivity or interference between Mouse INO80B and analogues was observed.
• Sequence Length: 345

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for INO80B elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate INO80B in samples. An antibody specific for INO80B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INO80B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INO80B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INO80B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: In Saccharomyces cerevisiae, orthologs of Tip49a and Tip49b, called Rvb1 and Rvb2, respectively, are subunits of two distinct ATP-dependent chromatin remodeling complexes, SWR1 and INO80. The mammalian Tip49a and Tip49b proteins are integral subunits of a chromatin remodeling complex bearing striking similarities to the S. cerevisiae SWR1 complex.A new mammalian Tip49a- and Tip49b-containing ATP-dependent chromatin remodeling complex, which includes orthologs of 8 of the 15 subunits of the S. cerevisiae INO80 chromatin remodeling complex as well as at least five additional subunits unique to the human INO80 (hINO80) complex. Finally, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.

NCBI and Uniprot Product Information

• NCBI GI #: 12963729
• NCBI GeneID: 70020
• NCBI Accession #: NP_076036.1
• NCBI GenBank Nucleotide #: NM_023547.1
• UniProt Accession #: Q99PT3; Q9CY38
• Molecular Weight: 40,565 Da
• NCBI Official Full Name: INO80 complex subunit B
• NCBI Official Synonym Full Names: INO80 complex subunit B
• NCBI Official Symbol: Ino80b
• NCBI Official Synonym Symbols: Papa1; Znhit4; HMG1YL4; Hmga1l4; 2510009I23Rik
• NCBI Protein Information: INO80 complex subunit B
• UniProt Protein Name: INO80 complex subunit B
• Protein Family: INO80 complex
• UniProt Gene Name: Ino80b
• UniProt Synonym Gene Names: Hmga1l4; Papa1; Znhit4; PAPA-1

Uniprot Description

Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.