Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit | BAT3 elisa kit

Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit

Cat. Num. AAA2021641

• Gene Names: BAG6; G3; BAT3; BAG-6; D6S52E
• Reactivity: Mouse
• Synonyms: HLA-B Associated Transcript 3 (BAT3); Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit; BAG6; Protein G3; Protein Scythe; BCL2-associated athanogene 6; BAG family molecular chaperone regulator 6; Large proline-rich protein BAG6; BAT3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HLA-B Associated Transcript 3 (BAT3).; No significant cross-reactivity or interference between HLA-B Associated Transcript 3 (BAT3) and analogues was observed.
• Sequence Length: 1,077

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAT3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B Associated Transcript 3 (BAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HLA-B Associated Transcript 3 (BAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HLA-B Associated Transcript 3 (BAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HLA-B Associated Transcript 3 (BAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BAT3 elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 13111925
• NCBI GeneID: 7917
• UniProt Accession #: P46379; O95874; A2ADJ7; A3KQ42; A3KQ44; A6NGY6; A6PWF7; B0UX84; B4DZ12; B4E3V4; E7EMZ4; F8VXY4
• Molecular Weight: 113,456 Da
• NCBI Official Full Name: HLA-B associated transcript 3
• NCBI Official Synonym Full Names: BCL2-associated athanogene 6
• NCBI Official Symbol: BAG6
• NCBI Official Synonym Symbols: G3; BAT3; BAG-6; D6S52E
• NCBI Protein Information: large proline-rich protein BAG6
• UniProt Protein Name: Large proline-rich protein BAG6
• Protein Family: Burkholderia TALE-like protein
• UniProt Gene Name: BAG6
• UniProt Synonym Gene Names: BAT3; G3; BAG-6; BAG6
• UniProt Entry Name: BAG6_HUMAN

NCBI Description

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BAT3: Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with L.pneumophila proteins Lpg2160 and LegU1. Interacts with NCR3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Ubiquitin conjugating system; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; ribosome binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: negative regulation of proteolysis; ubiquitin-dependent protein catabolic process; synaptonemal complex assembly; internal peptidyl-lysine acetylation; protein stabilization; immune system process; chromatin modification; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of cell proliferation; embryonic development; transport; spermatogenesis; brain development; cell differentiation; kidney development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of apoptosis; lung development