Mouse His (Histamine) ELISA Kit | His elisa kit

Mouse His (Histamine) ELISA Kit

Cat. Num. AAA7606341

• Reactivity: Mouse
• Synonyms: His (Histamine); Mouse His (Histamine) ELISA Kit; His elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HCY. No significant cross-reactivity or interference between HCY and analogues is observed.

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Competitive
• Detection Range: 7.8-500pmol/ml
• Sensitivity: <4.69pmol/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HCY were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HCY were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for His elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with HCY. During the reaction, HCY in the sample or standard competes with a fixed amount of HCY on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to HCY. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HCY in the samples is then determined by comparing the O D of the samples to the standard curve.