Mouse Hemopexin ELISA Kit | HPX elisa kit
Mouse Hemopexin ELISA Kit
Gene Names
HPX; HX; FLJ56652; HPX
Reactivity
Mouse
Synonyms
Hemopexin; Mouse Hemopexin ELISA Kit; Mouse Hemopexin; HPX elisa kit
Reactivity
Mouse
Samples
Immunoperoxidase Assay for Determination of Hemopexin in Mouse Samples
Assay Type
Sandwich
Intended Uses
The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in serum, plasma of mice.
Preparation and Storage
Store at 4 degree C.
Related Product Information for HPX elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure Hemopexin in serum, tissue extracts and other biological fluids.
Background: Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure Hemopexin in serum, tissue extracts and other biological fluids.
Product Categories/Family for HPX elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,676 Da
NCBI Official Full Name
hemopexin
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
HPX
NCBI Official Synonym Symbols
HX; FLJ56652; HPX
NCBI Protein Information
hemopexin; beta-1B-glycoprotein
UniProt Protein Name
Hemopexin
Protein Family
UniProt Gene Name
HPX
UniProt Entry Name
HEMO_HUMAN
NCBI Description
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq]
Uniprot Description
HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.5-p15.4
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; metal ion binding; heme transporter activity
Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; receptor-mediated endocytosis; positive regulation of tyrosine phosphorylation of Stat1 protein; heme transport; positive regulation of immunoglobulin production; viral reproduction; cellular iron ion homeostasis; hemoglobin metabolic process; heme metabolic process
Research Articles on HPX
1. Results indicate that for the discrimination of AD from ND control subjects, measurement of a set of markers including Abeta1-42, ApoA1 and HPX improved diagnostic performance over that obtained by measurement of Abeta1-42 alone.
2. In a family with a hypomaturation-type enamel defect, mutational and haplotype analyses revealed an amelogenesis imperfecta-causing point mutation in exon 6 of MMP20 that results in a single amino acid substitution in the hemopexin domain.
3. these observations result from the binding of heme in form beta with an orientation that differs from the crystallographically observed binding orientation for rabbit hemopexin
4. The structural consequences of metal ion binding to the form of hemopexin that dominates in plasma have been evaluated.
5. Hemopexin in minimal change nephropathy relapse subjects may exist in an altered isoform, showing enhanced protease activity as compared with subjects in remission, subjects with other forms of primary glomerulopathy, or healthy controls.
6. HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2
7. The conserved binding ability of the Hemopexin domains suggests that CD44 may act as a core molecule assembling multiple Membrane-type 1 matrix metalloproteinases on the cell surface.
8. Thermal denaturation of the human hemopexin-heme complex is investigated under a variety of solution conditions to identify factors that influence heme release, including the potential presence of transition metal ions or heme iron reduction.
9. The ability of hemopexin to bind metal ions raises the possibility that this protein may participate in the transport of metal ions in blood or in the exchange of metal ions between proteins.
10. Hemopexin and hemopexin domains of human proteins fulfill functions in activation of MMPs, inhibition of MMPs, dimerization, binding of substrates or ligands, cleavage of substrates, and endocytosis by LRP-1 and LRP-2.
11. hemopexin molecule as such can potentially act as a toxic protease, leading to proteinuria and glomerular alterations
2. In a family with a hypomaturation-type enamel defect, mutational and haplotype analyses revealed an amelogenesis imperfecta-causing point mutation in exon 6 of MMP20 that results in a single amino acid substitution in the hemopexin domain.
3. these observations result from the binding of heme in form beta with an orientation that differs from the crystallographically observed binding orientation for rabbit hemopexin
4. The structural consequences of metal ion binding to the form of hemopexin that dominates in plasma have been evaluated.
5. Hemopexin in minimal change nephropathy relapse subjects may exist in an altered isoform, showing enhanced protease activity as compared with subjects in remission, subjects with other forms of primary glomerulopathy, or healthy controls.
6. HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2
7. The conserved binding ability of the Hemopexin domains suggests that CD44 may act as a core molecule assembling multiple Membrane-type 1 matrix metalloproteinases on the cell surface.
8. Thermal denaturation of the human hemopexin-heme complex is investigated under a variety of solution conditions to identify factors that influence heme release, including the potential presence of transition metal ions or heme iron reduction.
9. The ability of hemopexin to bind metal ions raises the possibility that this protein may participate in the transport of metal ions in blood or in the exchange of metal ions between proteins.
10. Hemopexin and hemopexin domains of human proteins fulfill functions in activation of MMPs, inhibition of MMPs, dimerization, binding of substrates or ligands, cleavage of substrates, and endocytosis by LRP-1 and LRP-2.
11. hemopexin molecule as such can potentially act as a toxic protease, leading to proteinuria and glomerular alterations
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Product Notes
The Mouse HPX hpx (Catalog #AAA564072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564072 ELISA Kit recognizes Mouse HPX. It is sometimes possible for the material contained within the vial of "Hemopexin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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