Typical Testing Data/Standard Curve (for reference only)
(Mouse HAI-2/SPINT2 PicoKine ELISA Kit standard curve )
Mouse HAI-2/SPINT2 ELISA Kit | SPINT2 elisa kit
Mouse HAI-2/SPINT2 PicoKine ELISA Kit
Gene Names
Spint2; HAI-2; C76321; AL024025
Reactivity
Mouse
Synonyms
HAI-2/SPINT2; Mouse HAI-2/SPINT2 PicoKine ELISA Kit; Bikunin; placental; DIAR3; HAI2; HAI 2; HAI-2; Kop; PB; Placental bikunin; SPINT2; Q9WU03; Kunitz-type protease inhibitor 2; serine peptidase inhibitor; Kunitz type; 2; SPINT2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse HAI-2/SPINT2
Sequence Length
195
Samples
Cell culture supernates, serum, plasma(heparin, EDTA), cell lysates and tissue homogenates.
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse HAI-2/SPINT2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse HAI-2/SPINT2 PicoKine ELISA Kit standard curve )
Related Product Information for SPINT2 elisa kit
Principle of the Assay: Mouse HAI-2/SPINT2 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for HAI-2/SPINT2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: R30-K140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HAI-2/SPINT2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HAI-2/SPINT2 amount of sample captured in plate.
Background/Introduction: Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
Background/Introduction: Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,671 Da
NCBI Official Full Name
kunitz-type protease inhibitor 2 isoform b
NCBI Official Synonym Full Names
serine protease inhibitor, Kunitz type 2
NCBI Official Symbol
Spint2
NCBI Official Synonym Symbols
HAI-2; C76321; AL024025
NCBI Protein Information
kunitz-type protease inhibitor 2
UniProt Protein Name
Kunitz-type protease inhibitor 2
Protein Family
UniProt Gene Name
Spint2
UniProt Synonym Gene Names
Hai2; HAI-2
UniProt Entry Name
SPIT2_MOUSE
Uniprot Description
SPINT2: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3); also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Membrane protein, integral
Cellular Component: cytoplasm; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: establishment and/or maintenance of cell polarity; negative regulation of cell-cell adhesion; neural tube closure
Research Articles on SPINT2
Similar Products
Product Notes
The Mouse SPINT2 spint2 (Catalog #AAA178523) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178523 ELISA Kit recognizes Mouse SPINT2. It is sometimes possible for the material contained within the vial of "HAI-2/SPINT2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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