Typical Testing Data/Standard Curve (for reference only)
Mouse Glutamate carboxypeptidase 2 (FOLH1) ELISA Kit | FOLH1 elisa kit
Mouse Glutamate carboxypeptidase 2 (FOLH1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FNDC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FNDC1 antibody and an FNDC1-HRP conjugate. The assay sample and buffer are incubated together with FNDC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FNDC1 concentration since FNDC1 from samples and FNDC1-HRP conjugate compete for the anti-FNDC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FNDC1 from the sample, fewer sites are left to bind FNDC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FNDC1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]
Uniprot Description
FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.17.21; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: membrane; integral to plasma membrane; cytoplasm
Molecular Function: dipeptidase activity; peptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding
Biological Process: folic acid and derivative metabolic process; proteolysis
Research Articles on FOLH1
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Product Notes
The Mouse FOLH1 folh1 (Catalog #AAA7251595) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7251595 ELISA Kit recognizes Mouse FOLH1. It is sometimes possible for the material contained within the vial of "Glutamate carboxypeptidase 2 (FOLH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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