Mouse G Protein Beta 1 (GNb1) ELISA Kit | GNb1 elisa kit

Mouse G Protein Beta 1 (GNb1) ELISA Kit

Cat. Num. AAA453647

• Gene Names: Gnb1; Gnb-1; C77571; AA409223
• Reactivity: Mouse
• Synonyms: G Protein Beta 1 (GNb1); Mouse G Protein Beta 1 (GNb1) ELISA Kit; GN-B1; Guanine Nucleotide Binding Protein Beta 1; Transducin beta chain 1; GNb1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GNb1. No significant cross-reactivity or interference between GNb1 and analogues was observed.
• Sequence Length: 340

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.041ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GNb1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GNb1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNb1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GNb1 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GNb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GNb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GNb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GNb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 229608938
• NCBI GeneID: 14688
• NCBI Accession #: NP_001153488.1
• NCBI GenBank Nucleotide #: NM_001160016.1
• UniProt Accession #: P62874; P04697; P04901
• Molecular Weight: 37,377 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta 1
• NCBI Official Symbol: Gnb1
• NCBI Official Synonym Symbols: Gnb-1; C77571; AA409223
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: Gnb1

Uniprot Description

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction ().