Mouse Frataxin (FXN) ELISA Kit | FXN elisa kit

Mouse Frataxin (FXN) ELISA Kit

Cat. Num. AAA2704490

• Gene Names: FH; ATFH; F4C21.17; F4C21_17; frataxin homolog
• Reactivity: Mouse
• Synonyms: Frataxin (FXN); Mouse Frataxin (FXN) ELISA Kit; FA; CyaY; FARR; FRDA; X25; Friedreich ataxia protein; Frataxin intermediate form; Frataxin; mitochondrial; FXN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Frataxin (FXN).; No significant cross-reactivity or interference between Frataxin (FXN) and analogues was observed.
• Sequence Length: 187

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Frataxin (FXN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Frataxin (FXN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FXN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frataxin (FXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frataxin (FXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frataxin (FXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Frataxin (FXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42566266
• NCBI GeneID: 828013
• NCBI Accession #: NP_192233.2
• NCBI GenBank Nucleotide #: NM_116562.4
• UniProt Accession #: Q9ZR07; Q6GKV0
• Molecular Weight: 21,430 Da
• NCBI Official Full Name: frataxin-like protein
• NCBI Official Symbol: FH
• NCBI Official Synonym Symbols: ATFH; F4C21.17; F4C21_17; frataxin homolog
• NCBI Protein Information: frataxin-like protein
• UniProt Protein Name: Frataxin, mitochondrial
• Protein Family: Frataxin
• UniProt Gene Name: FH
• UniProt Synonym Gene Names: Fxn

NCBI Description

Encodes AtFH, a frataxin homolog. Frataxin is required for the biogenesis of mitochondria in different organisms. AtFH knock-out mutants are lethal. Required for full activity of mitochondrial Fe-S proteins. Deficiency of AtFH induces oxidative stress.

Uniprot Description

Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways (PubMed:17092311). May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+ (PubMed:17092311). May be able to store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization (PubMed:17092311). Binds to the mitochondrial cysteine desulfurase NIFS1 and increases its activity (PubMed:22511606).