Typical Testing Data/Standard Curve (for reference only)
(Mouse FOLR1 PicoKine ELISA Kit standard curve )
Mouse FOLR1 ELISA Kit | FOLR1 elisa kit
Mouse FOLR1 PicoKine ELISA Kit
Gene Names
Folr1; FBP1; Folbp1; Folbp-1
Reactivity
Mouse
Synonyms
FOLR1; Mouse FOLR1 PicoKine ELISA Kit; Folate receptor alpha; folate receptor 1 (adult); Adult folate binding protein; Adult folate-binding protein; FBP; Folate Receptor 1 Adult; Folate receptor 1; Folate Receptor 1 Precursor; Folate receptor adult; Folate receptor; FOLR; FOLR1_HUMAN; FR alpha; FR-alpha; FRalpha; KB cells FBP; MOV18; Ovarian cancer associated antigen; Ovarian tumor associated antigen; Ovarian tumor associated antigen MOv18; Ovarian tumor-associated antigen MOv18; FOLR1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse FOLR1
Sequence Length
255
Samples
Cell culture supernates, cell Iysates, tissue homo~lenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
46.9pg/ml-3000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse FOLR1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse FOLR1 PicoKine ELISA Kit standard curve )
Related Product Information for FOLR1 elisa kit
Principle of the Assay: The mouse FOLR1 EliSA Kit was based on standard sandwich en'<:'1me-linked immune-sorbent assay technology. A monoclonal aniibody from rat specific for FOLR 1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: T25-S232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FOLR1 amount of sample captured in plate.
Background/Introduction: Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.
Background/Introduction: Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,449 Da
NCBI Official Full Name
Folate receptor alpha
NCBI Official Synonym Full Names
folate receptor 1 (adult)
NCBI Official Symbol
Folr1
NCBI Official Synonym Symbols
FBP1; Folbp1; Folbp-1
NCBI Protein Information
folate receptor alpha; FR-alpha; folate binding protein 1; folate-binding protein 1
UniProt Protein Name
Folate receptor alpha
Protein Family
UniProt Gene Name
Folr1
UniProt Synonym Gene Names
Fbp1; Folbp1; FR-alpha
UniProt Entry Name
FOLR1_MOUSE
Uniprot Description
FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family.
Protein type: Membrane protein, GPI anchor
Cellular Component: anchored to external side of plasma membrane; anchored to plasma membrane; cell surface; membrane; cell; brush border membrane; extracellular region; plasma membrane; intracellular; cytoplasmic vesicle; brush border; endosome
Molecular Function: folic acid transporter activity; receptor activity; folic acid binding
Biological Process: transport; folic acid metabolic process; posttranslational protein targeting to membrane; folic acid transport
Research Articles on FOLR1
Similar Products
Product Notes
The Mouse FOLR1 folr1 (Catalog #AAA177151) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177151 ELISA Kit recognizes Mouse FOLR1. It is sometimes possible for the material contained within the vial of "FOLR1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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