Mouse Fibulin 3 (FBLN3) ELISA Kit | FBLN3 elisa kit

Mouse Fibulin 3 (FBLN3) ELISA Kit

Cat. Num. AAA454777

• Reactivity: Mouse
• Synonyms: Fibulin 3 (FBLN3); Mouse Fibulin 3 (FBLN3) ELISA Kit; EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5; FBLN3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FBLN3. No significant cross-reactivity or interference between FBLN3 and analogues was observed.
• Sequence Length: 493

• Samples: Serum, plasma, urine and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 6.6pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FBLN3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FBLN3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FBLN3 in mouse serum, plasma, urine and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBLN3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FBLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FBLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FBLN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 164607147
• NCBI GeneID: 216616
• NCBI Accession #: NP_666127.2
• NCBI GenBank Nucleotide #: NM_146015.2
• UniProt Accession #: Q8BPB5; Q6Y3N6
• Molecular Weight: 54,953 Da
• NCBI Official Full Name: EGF-containing fibulin-like extracellular matrix protein 1
• NCBI Official Synonym Full Names: epidermal growth factor-containing fibulin-like extracellular matrix protein 1
• NCBI Official Symbol: Efemp1
• NCBI Protein Information: EGF-containing fibulin-like extracellular matrix protein 1
• UniProt Protein Name: EGF-containing fibulin-like extracellular matrix protein 1
• UniProt Gene Name: Efemp1
• UniProt Synonym Gene Names: Fbln3; FIBL-3

Uniprot Description

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth ().