Mouse F-box only protein 6 (FBXO6) ELISA Kit | FBXO6 elisa kit

Mouse F-box only protein 6 (FBXO6) ELISA Kit

Cat. Num. AAA7208168

• Gene Names: FBXO6; FBG2; FBS2; FBX6; Fbx6b
• Reactivity: Mouse
• Synonyms: F-box only protein 6 (FBXO6); Mouse F-box only protein 6 (FBXO6) ELISA Kit; FBXO6 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXO6 elisa kit

Intended Uses: This FBXO6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FBXO6. This ELISA kit for research use only!

Principle of the Assay: FBXO6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FBXO6 antibody and an FBXO6-HRP conjugate. The assay sample and buffer are incubated together with FBXO6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO6 concentration since FBXO6 from samples and FBXO6-HRP conjugate compete for the anti-FBXO6 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO6 from the sample, fewer sites are left to bind FBXO6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBXO6 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 8922188
• NCBI GeneID: 26270
• NCBI Accession #: NP_060908.1
• NCBI GenBank Nucleotide #: NM_018438.5
• Molecular Weight: 33,933 Da
• NCBI Official Full Name: F-box only protein 6
• NCBI Official Synonym Full Names: F-box protein 6
• NCBI Official Symbol: FBXO6
• NCBI Official Synonym Symbols: FBG2; FBS2; FBX6; Fbx6b
• NCBI Protein Information: F-box only protein 6; F-box protein FBG2; F-box protein Fbx6; F-box/G-domain protein 2; F-box protein that recognizes sugar chains 2
• UniProt Protein Name: F-box only protein 6
• UniProt Gene Name: FBXO6
• UniProt Synonym Gene Names: FBG2; FBS2; FBX6
• UniProt Entry Name: FBX6_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class, and its C-terminal region is highly similar to that of rat NFB42 (neural F Box 42 kDa) which may be involved in the control of the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO6: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex- type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; carbohydrate binding; glycoprotein binding

Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; DNA damage checkpoint; protein ubiquitination; glycoprotein catabolic process; DNA repair; proteolysis; response to unfolded protein