Mouse E3 ubiquitin-protein ligase AMFR (AMFR) ELISA Kit | AMFR elisa kit

Mouse E3 ubiquitin-protein ligase AMFR (AMFR) ELISA Kit

Cat. Num. AAA287837

• Gene Names: Amfr; gp78
• Reactivity: Mouse
• Synonyms: E3 ubiquitin-protein ligase AMFR (AMFR); Mouse E3 ubiquitin-protein ligase AMFR (AMFR) ELISA Kit; GP78; RNF45; AMFR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse AMFR. No significant cross-reactivity or interference between Mouse AMFR and analogues was observed.
• Sequence Length: 639

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for AMFR elisa kit

This assay employs a two-site sandwich ELISA to quantitate AMFR in samples. An antibody specific for AMFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 113205073
• NCBI GeneID: 23802
• NCBI Accession #: NP_035917.2
• NCBI GenBank Nucleotide #: NM_011787.2
• UniProt Accession #: Q9R049; Q8K008; Q99LH5
• Molecular Weight: 72,753 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase AMFR
• NCBI Official Synonym Full Names: autocrine motility factor receptor
• NCBI Official Symbol: Amfr
• NCBI Official Synonym Symbols: gp78
• NCBI Protein Information: E3 ubiquitin-protein ligase AMFR
• UniProt Protein Name: E3 ubiquitin-protein ligase AMFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Amfr
• UniProt Synonym Gene Names: AMF receptor

Uniprot Description

E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis ().