Mouse Discs, Large Homolog 4 (DLG4) ELISA Kit | DLG4 elisa kit

Mouse Discs, Large Homolog 4 (DLG4) ELISA Kit

Cat. Num. AAA2019506

• Gene Names: DLG4; PSD95; SAP90; SAP-90
• Reactivity: Mouse
• Synonyms: Discs; Large Homolog 4 (DLG4); Mouse Discs; Large Homolog 4 (DLG4) ELISA Kit; PSD95; SAP90; Postsynaptic density protein 95; Synapse-associated protein 90; DLG4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DLG4. No significant cross-reactivity or interference between DLG4 and analogues was observed.
• Sequence Length: 767

• Samples: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: .156-10ng/mL
• Sensitivity: 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DLG4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DLG4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DLG4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DLG4 in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DLG4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DLG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DLG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DLG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DLG4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4557529
• NCBI GeneID: 1742
• NCBI Accession #: NP_001356.1
• NCBI GenBank Nucleotide #: NM_001365.3
• UniProt Accession #: P78352; Q92941; Q9UKK8; B7Z1S1; G5E939
• Molecular Weight: 80,125 Da
• NCBI Official Full Name: disks large homolog 4 isoform 1
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: DLG4
• NCBI Official Synonym Symbols: PSD95; SAP90; SAP-90
• NCBI Protein Information: disks large homolog 4
• UniProt Protein Name: Disks large homolog 4
• Protein Family: Disks large
• UniProt Gene Name: DLG4
• UniProt Synonym Gene Names: PSD95; PSD-95; SAP-90; SAP90
• UniProt Entry Name: DLG4_HUMAN

NCBI Description

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: dendrite cytoplasm; cortical cytoskeleton; voltage-gated potassium channel complex; basolateral plasma membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; guanylate kinase activity; ionotropic glutamate receptor binding; protein binding; beta-1 adrenergic receptor binding; protein complex binding; acetylcholine receptor binding; D1 dopamine receptor binding; protein phosphatase binding; PDZ domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; nervous system development; axon guidance; synaptic vesicle maturation; social behavior; learning; signal transduction; positive regulation of synaptic transmission; establishment and/or maintenance of epithelial cell polarity; synaptic transmission; elevation of cytosolic calcium ion concentration; establishment of protein localization; protein complex assembly; neuromuscular process controlling balance; nucleotide phosphorylation; negative regulation of receptor internalization