Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit | DLD elisa kit

Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

Cat. Num. AAA9717868

• Reactivity: Mouse
• Synonyms: Dihydrolipoyl Dehydrogenase (DLD); Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit; DLD; tcag7.39; DLDH; E3; GCSL; LAD; PHE3; E3 component of pyruvate dehydrogenase complex; 2-oxo-glutarate complex; branched chain keto acid dehydrogenase complex; OTTHUMP00000206749; diaphorase; dihydrolipo; DLD elisa kit

• Reactivity: Mouse
• Specificity: Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLD. No significant cross-reactivity or interference between Mouse DLD and analogues was observed.
• Sequence Length: 509

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Dihydrolipoyl dehydrogenase (DLD) Standard Curve.)

Related Product Information for DLD elisa kit

Description: This Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit employs a two-site sandwich ELISA to quantitate DLD in samples. An antibody specific for DLD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The dihydrolipoate, still bound to a lysine residue of the complex, then migrates to the dihydrolipoyl dehydrogenase (E3) active site where it undergoes a flavin-mediated oxidation, identical in chemistry to disulfide isomerase. First, FAD oxidizes dihydrolipoate back to its lipoate resting state, producing FADH2. Dihydrolipoamide dehydrogenase is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 31982856
• NCBI GeneID: 13382
• NCBI Accession #: NP_031887.2
• NCBI GenBank Nucleotide #: NP_031887.2
• UniProt Accession #: O08749
• NCBI Official Full Name: dihydrolipoyl dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: dihydrolipoamide dehydrogenase
• NCBI Official Symbol: Dld
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• UniProt Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein
• UniProt Gene Name: Dld

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. [provided by RefSeq, Jan 2014]

Uniprot Description

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of the alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form has additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction ().