Typical Testing Data/Standard Curve (for reference only)
Mouse Destrin (DSTN) ELISA Kit | DSTN elisa kit
Mouse Destrin (DSTN) ELISA Kit
Reactivity
Mouse
Synonyms
Destrin (DSTN); Mouse Destrin (DSTN) ELISA Kit; DSTN elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of DSTN. No significant cross-reactivity or interference between DSTN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DSTN and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DSTN elisa kit
Intended Uses: This DSTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DSTN. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: DSTN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DSTN antibody and an DSTN-HRP conjugate. The assay sample and buffer are incubated together with DSTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSTN concentration since DSTN from samples and DSTN-HRP conjugate compete for the anti-DSTN antibody binding site. Since the number of sites is limited, as more sites are occupied by DSTN from the sample, fewer sites are left to bind DSTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSTN concentration in each sample is interpolated from this standard curve.
Principle of the Assay: DSTN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DSTN antibody and an DSTN-HRP conjugate. The assay sample and buffer are incubated together with DSTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSTN concentration since DSTN from samples and DSTN-HRP conjugate compete for the anti-DSTN antibody binding site. Since the number of sites is limited, as more sites are occupied by DSTN from the sample, fewer sites are left to bind DSTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSTN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DSTN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,506 Da
NCBI Official Full Name
destrin
NCBI Official Symbol
DSTN
NCBI Protein Information
destrin; ADF; actin-depolymerizing factor
UniProt Protein Name
Destrin
Protein Family
UniProt Gene Name
DSTN
UniProt Synonym Gene Names
DSN; ADF
UniProt Entry Name
DEST_PIG
Uniprot Description
Destrin: an actin-depolymerizing cytoskeletal protein that belongs to the ADF actin-binding protein family. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Present in many vertebrate tissues and particularly abundant in neuronal cells. It binds F-actin in a pH-dependent manner which affects it's severing/depolymerizing activities. It also binds G-actin at pH 8.0 thereby sequestering monomers and preventing polymerization.
Protein type: Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20p12.1
Cellular Component: cortical actin cytoskeleton; actin cytoskeleton
Molecular Function: actin binding
Biological Process: actin filament severing; actin filament depolymerization; positive regulation of actin filament depolymerization; actin polymerization and/or depolymerization
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Product Notes
The Mouse DSTN dstn (Catalog #AAA7238288) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238288 ELISA Kit recognizes Mouse DSTN. It is sometimes possible for the material contained within the vial of "Destrin (DSTN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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