Typical Testing Data/Standard Curve (for reference only)
Mouse Delta-type opioid receptor (OPRD1) ELISA Kit | OPRD1 elisa kit
Mouse Delta-type opioid receptor (OPRD1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: OPRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OPRD1 antibody and an OPRD1-HRP conjugate. The assay sample and buffer are incubated together with OPRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPRD1 concentration since OPRD1 from samples and OPRD1-HRP conjugate compete for the anti-OPRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OPRD1 from the sample, fewer sites are left to bind OPRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPRD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
DOR-1: delta opioid receptor, a G-protein coupled receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. Receptor for enkephalins.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1
Chromosomal Location of Human Ortholog: 1p36.1-p34.3
Cellular Component: postsynaptic membrane; neuron projection; integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; intrinsic to plasma membrane; vesicle; lipid raft
Molecular Function: neuropeptide binding; protein binding; opioid receptor activity
Biological Process: adult locomotory behavior; activation of CREB transcription factor; regulation of calcium ion transport; sensory perception of pain; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of protein oligomerization; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of mitochondrial membrane potential; protein import into nucleus, translocation; neuropeptide signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway
Research Articles on OPRD1
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Product Notes
The Mouse OPRD1 oprd1 (Catalog #AAA7234849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234849 ELISA Kit recognizes Mouse OPRD1. It is sometimes possible for the material contained within the vial of "Delta-type opioid receptor (OPRD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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