Mouse Delta-type opioid receptor (OPRD1) ELISA Kit | OPRD1 elisa kit

Mouse Delta-type opioid receptor (OPRD1) ELISA Kit

Cat. Num. AAA7234849

• Gene Names: OPRD1; OPRD
• Reactivity: Mouse
• Synonyms: Delta-type opioid receptor (OPRD1); Mouse Delta-type opioid receptor (OPRD1) ELISA Kit; OPRD1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of OPRD1. No significant cross-reactivity or interference between OPRD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPRD1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPRD1 elisa kit

Intended Uses: This OPRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OPRD1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: OPRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OPRD1 antibody and an OPRD1-HRP conjugate. The assay sample and buffer are incubated together with OPRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPRD1 concentration since OPRD1 from samples and OPRD1-HRP conjugate compete for the anti-OPRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OPRD1 from the sample, fewer sites are left to bind OPRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPRD1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OPRD1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 63477962
• NCBI GeneID: 4985
• NCBI Accession #: NP_000902.3
• NCBI GenBank Nucleotide #: NM_000911.3
• Molecular Weight: 40,369 Da
• NCBI Official Full Name: delta-type opioid receptor
• NCBI Official Synonym Full Names: opioid receptor, delta 1
• NCBI Official Symbol: OPRD1
• NCBI Official Synonym Symbols: OPRD
• NCBI Protein Information: delta-type opioid receptor; DOR-1; D-OR-1; delta opioid receptor 1
• UniProt Protein Name: Delta-type opioid receptor
• Protein Family: Delta-type opioid receptor
• UniProt Gene Name: OPRD1
• UniProt Synonym Gene Names: OPRD; D-OR-1; DOR-1
• UniProt Entry Name: OPRD_HUMAN

Uniprot Description

DOR-1: delta opioid receptor, a G-protein coupled receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. Receptor for enkephalins.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p34.3

Cellular Component: postsynaptic membrane; neuron projection; integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; intrinsic to plasma membrane; vesicle; lipid raft

Molecular Function: neuropeptide binding; protein binding; opioid receptor activity

Biological Process: adult locomotory behavior; activation of CREB transcription factor; regulation of calcium ion transport; sensory perception of pain; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of protein oligomerization; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of mitochondrial membrane potential; protein import into nucleus, translocation; neuropeptide signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway