Mouse Cystatin A (CSTA) ELISA Kit | CSTA elisa kit

Mouse Cystatin A (CSTA) ELISA Kit

Cat. Num. AAA2024659

• Gene Names: CSTA; AREI; STF1; STFA
• Reactivity: Mouse
• Synonyms: Cystatin A (CSTA); Mouse Cystatin A (CSTA) ELISA Kit; Cys-A; STF1; STFA; Stefin A; Cystatin-AS; CSTA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cystatin A (CSTA).; No significant cross-reactivity or interference between Cystatin A (CSTA) and analogues was observed.
• Sequence Length: 98

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Saliva, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 25.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin A (CSTA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin A (CSTA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSTA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin A (CSTA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin A (CSTA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin A (CSTA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin A (CSTA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CSTA elisa kit

Tumor immunity; Infection immunity; Pulmonology

NCBI and Uniprot Product Information

• NCBI GI #: 4885165
• NCBI GeneID: 1475
• NCBI Accession #: NP_005204.1
• NCBI GenBank Nucleotide #: NM_005213.3
• UniProt Accession #: P01040; Q6IB90
• Molecular Weight: 11,006 Da
• NCBI Official Full Name: cystatin-A
• NCBI Official Synonym Full Names: cystatin A (stefin A)
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; STF1; STFA
• NCBI Protein Information: cystatin-A
• UniProt Protein Name: Cystatin-A
• Protein Family: Cystatin
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA
• UniProt Entry Name: CYTA_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CSTA: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Defects in CSTA are the cause of ichthyosis exfoliative autosomal recessive ichthyosis bullosa of Siemens-like (AREI). A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non- erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes. Belongs to the cystatin family.

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cornified envelope; nucleus

Molecular Function: protein binding, bridging; protease binding; cysteine protease inhibitor activity; structural molecule activity

Biological Process: negative regulation of proteolysis; keratinocyte differentiation; cell-cell adhesion; negative regulation of peptidase activity; peptide cross-linking

Disease: Exfoliative Ichthyosis, Autosomal Recessive, Ichthyosis Bullosa Of Siemens-like