Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit | cGMP elisa kit

Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit

Cat. Num. AAA267678

• Gene Names: PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
• Reactivity: Mouse
• Synonyms: Cyclic Guanosine Monophosphate (cGMP); Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit; cGMP elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 671

• Samples: Mouse serum, plasma or cell culture supernatant
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/mL
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for cGMP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse cGMP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 148612818
• NCBI GeneID: 5592
• NCBI Accession #: NP_001091982.1
• NCBI GenBank Nucleotide #: NM_001098512.2
• Molecular Weight: 44,185 Da
• NCBI Official Full Name: cGMP-dependent protein kinase 1 isoform 1
• NCBI Official Synonym Full Names: protein kinase, cGMP-dependent, type I
• NCBI Official Symbol: PRKG1
• NCBI Official Synonym Symbols: 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
• NCBI Protein Information: cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, regulatory, type I, beta
• UniProt Protein Name: cGMP-dependent protein kinase 1
• UniProt Gene Name: PRKG1
• UniProt Synonym Gene Names: PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI
• UniProt Entry Name: KGP1_HUMAN

NCBI Description

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

Uniprot Description

PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.12; AGC group; PKG family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; calcium channel regulator activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding

Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; neuron migration; dendrite development; signal transduction; blood coagulation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation