Mouse Clathrin light chain A (CLTA) ELISA Kit | CLTA elisa kit

Mouse Clathrin light chain A (CLTA) ELISA Kit

Cat. Num. AAA287761

• Gene Names: Clta; Lca; AV026556
• Reactivity: Mouse
• Synonyms: Clathrin light chain A (CLTA); Mouse Clathrin light chain A (CLTA) ELISA Kit; LCA; clathrin; light polypeptide (Lca); light polypeptide A; CLTA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CLTA. No significant cross-reactivity or interference between Mouse CLTA and analogues was observed.
• Sequence Length: 235

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CLTA elisa kit

This assay employs a two-site sandwich ELISA to quantitate CLTA in samples. An antibody specific for CLTA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLTA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLTA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLTA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 408360022
• NCBI GeneID: 12757
• UniProt Accession #: O08585
• Molecular Weight: 25,604 Da
• NCBI Official Full Name: Clathrin light chain A
• NCBI Official Synonym Full Names: clathrin, light polypeptide (Lca)
• NCBI Official Symbol: Clta
• NCBI Official Synonym Symbols: Lca; AV026556
• NCBI Protein Information: clathrin light chain A
• UniProt Protein Name: Clathrin light chain A
• Protein Family: Clathrin
• UniProt Gene Name: Clta
• UniProt Synonym Gene Names: Lca

NCBI Description

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge ().