Mouse Cerberus (CER1) ELISA Kit | CER1 elisa kit

Mouse Cerberus (CER1) ELISA Kit

Cat. Num. AAA9718106

• Gene Names: Cer1; Cerl; Cerl1; Cerr1; cer-1
• Reactivity: Mouse
• Synonyms: Cerberus (CER1); Mouse Cerberus (CER1) ELISA Kit; CER1; DAND4; MGC119894; MGC119895; MGC96951; cerberus 1; cerberus-related 1; CER1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Cerberus (CER1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CER1. No significant cross-reactivity or interference between Mouse CER1 and analogues was observed.
• Sequence Length: 272

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Cerberus (CER1) Standard Curve.)

Related Product Information for CER1 elisa kit

Description: This Mouse Cerberus (CER1) ELISA Kit employs a two-site sandwich ELISA to quantitate CER1 in samples. An antibody specific for CER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Cerberus also known as CER1 is a cytokine member of the cystine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/ Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.Cerberus is an inhibitor in the TGF beta signaling pathway secreted during the gastrulation phase of the embryogenesis.Cer1 mimicked the anti-BMP activity of Xcer in Xenopus animal caps, although with reduced potency. In situ hybridization to early gastrula mouse embryos revealed that Cer1 expression was restricted to the anterior primitive endoderm, the tissue thought to initiate head induction.

NCBI and Uniprot Product Information

• NCBI GI #: 6753410
• NCBI GeneID: 12622
• NCBI Accession #: NP_034017.1
• NCBI GenBank Nucleotide #: NP_034017.1
• UniProt Accession #: O55233
• NCBI Official Full Name: cerberus
• NCBI Official Synonym Full Names: cerberus 1, DAN family BMP antagonist
• NCBI Official Symbol: Cer1
• NCBI Official Synonym Symbols: Cerl; Cerl1; Cerr1; cer-1
• NCBI Protein Information: cerberus
• UniProt Protein Name: Cerberus
• Protein Family: Cerberus
• UniProt Gene Name: Cer1
• UniProt Synonym Gene Names: Cerl; Cerr1; Cer-l

Uniprot Description

Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak.