Typical Testing Data/Standard Curve (for reference only)
Mouse Cell division cycle protein 123 homolog (CDC123) ELISA Kit | CDC123 elisa kit
Mouse Cell division cycle protein 123 homolog (CDC123) ELISA Kit
Gene Names
CDC123; D123; C10orf7
Reactivity
Mouse
Synonyms
Cell division cycle protein 123 homolog (CDC123); Mouse Cell division cycle protein 123 homolog (CDC123) ELISA Kit; CDC123 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.png/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CDC123 elisa kit
Intended Uses||This CDC123 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CDC123. This ELISA kit for research use only!
Principle of the Assay: CDC123 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC123 antibody and an CDC123-HRP conjugate. The assay sample and buffer are incubated together with CDC123-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theCDC123 concentration since CDC123 from samples and CDC123-HRP conjugate compete for the anti-CDC123 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC123 from the sample, fewer sites are left to bind CDC123-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC123 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CDC123 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC123 antibody and an CDC123-HRP conjugate. The assay sample and buffer are incubated together with CDC123-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theCDC123 concentration since CDC123 from samples and CDC123-HRP conjugate compete for the anti-CDC123 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC123 from the sample, fewer sites are left to bind CDC123-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC123 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CDC123 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,135 Da
NCBI Official Full Name
cell division cycle protein 123 homolog
NCBI Official Synonym Full Names
cell division cycle 123
NCBI Official Symbol
CDC123
NCBI Official Synonym Symbols
D123; C10orf7
NCBI Protein Information
cell division cycle protein 123 homolog; PZ32; HT-1080; cell division cycle 123 homolog
UniProt Protein Name
Cell division cycle protein 123 homolog
Protein Family
UniProt Gene Name
CDC123
UniProt Synonym Gene Names
C10orf7; D123; Protein D123
UniProt Entry Name
CD123_HUMAN
Uniprot Description
CDC123: Required for S phase entry of the cell cycle. Belongs to the CDC123 family.
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: cytoplasm
Biological Process: cell division; positive regulation of cell proliferation; regulation of mitotic cell cycle; cell cycle arrest
Research Articles on CDC123
Similar Products
Product Notes
The Mouse CDC123 cdc123 (Catalog #AAA7229547) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7229547 ELISA Kit recognizes Mouse CDC123. It is sometimes possible for the material contained within the vial of "Cell division cycle protein 123 homolog (CDC123), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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