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Typical Testing Data/Standard Curve (for reference only)

Mouse CD20 (B-lymphocyte antigen CD20) ELISA Kit | CD20 elisa kit

Mouse CD20 (B-lymphocyte antigen CD20) ELISA Kit

Gene Names
Ms4a1; Cd20; Ly-44; Ms4a2; AA960661
Reactivity
Mouse
Synonyms
CD20 (B-lymphocyte antigen CD20); Mouse CD20 (B-lymphocyte antigen CD20) ELISA Kit; B-lymphocyte antigen CD20; B-cell differentiation antigen Ly-44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; CD20; Ms4a1; Ly-44; Ms4a2; CD20 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CD20. No significant cross-reactivity or interference between CD20 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD20 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,958 Da
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
Ms4a1
NCBI Official Synonym Symbols
Cd20; Ly-44; Ms4a2; AA960661
NCBI Protein Information
B-lymphocyte antigen CD20
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
Ms4a1
UniProt Synonym Gene Names
Cd20; Ly-44; Ms4a2

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Cell surface; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19 A|19 8.19 cM

Cellular Component: external side of plasma membrane; extracellular exosome; extracellular space; integral component of membrane; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: epidermal growth factor receptor binding; protein binding

Biological Process: B cell activation

Research Articles on CD20

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Product Notes

The Mouse CD20 ms4a1 (Catalog #AAA7612377) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7612377 ELISA Kit recognizes Mouse CD20. It is sometimes possible for the material contained within the vial of "CD20 (B-lymphocyte antigen CD20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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