Mouse Cardiac Troponin I (cTnI) ELISA Kit | cTnI elisa kit

Mouse Cardiac Troponin I (cTnI) Mini Samples ELISA Kit

Cat. Num. AAA2708301

• Gene Names: Tnni3; Tn1; cTnI
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Cardiac Troponin I (cTnI); Mouse Cardiac Troponin I (cTnI) Mini Samples ELISA Kit; Cardiac Troponin I; TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac; cTnI elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Cardiac Troponin I (cTnI).; No significant cross-reactivity or interference between Mini Samples Cardiac Troponin I (cTnI) and analogues was observed.
• Sequence Length: 211

• Applicable Applications for cTnI elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Cardiac Troponin I (cTnI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Cardiac Troponin I (cTnI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Cardiac Troponin I (cTnI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Cardiac Troponin I (cTnI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6678393
• NCBI GeneID: 21954
• NCBI Accession #: NP_033432.1
• NCBI GenBank Nucleotide #: NP_033432.1
• UniProt Accession #: P48787
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I, cardiac 3
• NCBI Official Symbol: Tnni3
• NCBI Official Synonym Symbols: Tn1; cTnI
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• UniProt Gene Name: Tnni3
• UniProt Entry Name: TNNI3_MOUSE

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motor; Motility/polarity/chemotaxis

Cellular Component: contractile fiber; cytoplasm; myofibril; sarcomere; troponin complex

Molecular Function: actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; negative regulation of ATPase activity; regulation of muscle contraction; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; striated muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis